Tips on doing with protein

我这里好些人都是把蛋白在液氮里冻成小珠子然后放-80的， 用的时候拿一颗。
就是反复冻融是不行的。

那个得小心小珠子沾一起
不过有的的确很脆弱
我的一个酶冻融第一次可以，第二次就over

加甘油可以增加蛋白的稳定性，浓度一般5%-50%，取决于蛋白的性质。
当然也不是一定要放的，有时后续实验里不能有甘油。





Well, I was reading this article by Markram in Nature today. He opened with
the setence, industrialization hit the biological sciences near the turn of
21st century. I think this whole enterprise will look very different in 10-
20 years.
So China could indeed take the labor intensive biology idea and run with it.
I predict that all labor intensive biology will be outsourced to China in
20 years just like manufacturing jobs are outsourced to China now.
High throughput experiments will generate the leads and follow-up
experiments using human labor will be done in China on a massive scale. I
mean, what keeps current biology expensive, namely all those patents etc
will expire soon. Reagents will be cheap and humans will be expensive. So
the key is to build the infrastructure companies in China now to supply
those reagents etc. That will be where I put my money. That's my bold
prediction. :)